FOXP3 antibody (PE-FineTest®594)

Catalog No. ABIN7675504

The Rat Monoclonal anti-FOXP3 antibody has been validated for FACS. It is suitable to detect FOXP3 in samples from Mouse and Rat.

Quick Overview for FOXP3 antibody (PE-FineTest®594) (ABIN7675504)

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Reactivity: Mouse, Rat

Host: Rat

Clonality: Monoclonal

Conjugate: This FOXP3 antibody is conjugated to PE-FineTest®594

Application: Flow Cytometry (FACS)

Clone: FJK-16s

Product Details anti-FOXP3 Antibody (PE-FineTest®594)

Purpose: PE-FineTest®594 Anti-Mouse/Rat Foxp3 Antibody

Isotype: IgG2a, kappa

Application Details

Application Notes: Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 μL staining volume or per 100 μL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

Comment:

PE-FineTest®594 is designed to be excited by the blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 620 nm (e.g., a 610/20 nm bandpass filter).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0. 1 % sodium azide, 1 %BSA, pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: 4 °C

Storage Comment: 2-8°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Alternative Name: Foxp3

Background:

Synonyms: Forkhead box protein P3|Scurfin|JM2|IPEX|Zinc finger protein JM2

Background: FOXP3 is a 47 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4⁺/CD25^- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity.

Molecular Weight: 0

Gene ID: 20371

UniProt: Q99JB6

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation